詳細介紹
品牌 | 其他品牌 | 規格 | Ag0032 |
---|---|---|---|
供貨周期 | 一周 | 應用領域 | 醫療衛生,生物產業 |
Recombinant Human 4Ig-B7-H3 Protein,His-tag
重組人B7-H3蛋白,His-tag
Recombinant Human CD276 Protein,His-tag
重組人CD276蛋白,His-tag
PRODUCT DETAILS | ||||
Alternative Names | CD276, B7-H3, B7H3 | |||
Expression System | HEK 293 cells | |||
Purity | ≥95% SDS-PAGE | |||
Protein Length | Protein Leu29-Ala534, with a C-terminal 6-His tag. Database link: uniprot.org/uniprot/Q5ZPR3 | |||
Accession# | NP_079516 | |||
Predicted Molecular Mass | 55 kDa | |||
SDS-PAGE | 70-90 kDa | |||
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with trehalose | |||
Reconstitution | Centrifuge vial prior to opening vial. Add 0.5 mL sterile water or 0.01M PBS (pH7.2) at 0.4 mg/mL Gently pipet solution down the sides of the vial. DO NOT VORTEX. | |||
Please note: For research use only. Not for diagnostic or therapeutic use. | ||||
SHIPPING & STROAGE | ||||
Shipping | Lyophilized product is shipped at ambient temperature. | |||
Storage | Upon receipt, store immediately at -20 to -70°C:12 months from date of receipt. After reconstitution under sterile conditions: 2 to 8°C or 1 month or -20 to -70°C for 6 months. Avoid freeze-thaw cycles. | |||
APPLICATIONS | ||||
Item | Description | Data Examples | ||
SDS PAGE | 10 µg recombinant human 4Ig-B7-H3(Cat.#: 0721141), his tag protein, reducing condition |
074A031 Recombinant human B7H3 His Tag
074A032 Recombinant human B7H3 His Tag
074A033 Recombinant human B7H3 human Fc
074A034 Recombinant human B7H3 human Fc
074A035 Recombinant human B7H3 mouse Fc
074A036 Recombinant human B7H3 mouse Fc
北京匯智泰康醫藥技術有限公司致力于為藥物、醫療器械、化妝品、新化學物質、農藥、消毒產品等生產研發機構提供相關產品與服務。公司取得的資質包括 CNAS,GLP,CMA,AAALAC,化學品毒性鑒定機構等。
原核表達系統:
將克隆化基因插入合適載體后導入原核生物用于表達大量蛋白質的方法一般稱為原核表達。主要包括:大腸桿菌表達系統、枯草芽孢桿菌表達系統、鏈霉菌表達系統等,其中應用廣泛的是大腸桿菌表達系統。
酵母表達 :
酵母表達系統是研究真核蛋白質表達和分析的有力工具,擁有轉錄后加工修飾功能,適合于穩定表達有功能的外源蛋白質。與昆蟲表達系統和哺乳動物表達系統相比,酵母表達系統操作簡便,成本低廉,可大規模進行發酵。
哺乳動物細胞表達:
哺乳動物細胞表達系統的優勢在于能夠指導蛋白質的正確折疊,提供復雜的N型糖基化和準確的O型糖基化等多種翻譯后加工功能,因而表達產物在分子結構、理化特性和生物學功能方面接近于天然的高等生物蛋白質分子。
目前已有許多重要的蛋白及糖蛋白利用哺乳動物細胞表達系統大量制備、生產。如:人組織型血纖蛋白酶原激活因子、凝血因子Ⅷ、干擾素、乙肝表面抗原、紅血球生成激素、人生長激素等。
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